Protocol: TruSight Tumor 170 DNA Shearing Quick Guide
A validated protocol with Illumina using the Covaris ME220 and microTUBE-50 strip.
App Note: Variability in DNA Fragment Size and Distribution Analysis Across Various Fragment Analyzers.
This application note addresses the observed bias of the determination of DNA fragment size and distribution among popular analytical instruments (Fragment Analyzer, Bioanalyzer, and TapeStation) when Tris-EDTA (TE) is used as shearing buffer.
Updated Protocol: truChIP Chromatin Shearing Kit
Note: Please follow this protocol if you purchased your kit after November 13, 2019.