AFA-based Sputum Liquification to Enable Downstream Microbial Analysis
Adaptive Focused Acoustics is well established for shearing DNA for Next Generation Sequencing and for extraction of peptides prior to MALDI-TOF MS. These applications are optimized by considering the initial starting material concentrations, volumes and desired outcomes. Then, a specific software-controlled program allows the researcher to conduct experiments with Peak Incident Power (PIP), Duty Factor (DF), Cycles per Burst (CPB), temperature control and process duration. Following recommendations from Covaris, a research Lab can optimize an extraction protocol by measuring changes in results while controlling a single variable. After each variable is considered, a final set of conditions can be locked in for reproducible results.