AFA-based RNA Extraction from Mycobacteria smegmatis
We demonstrate that intact RNA can be efficiently extracted from Mycobacteria smegmatis using the Covaris Adaptive Focused Acoustics® (AFA®) M220 or ME220 Focused-ultrasonicators. By applying precise control of AFA power conditions, RNA can be differentially extracted to favor intact subunits or shorter fragments. AFA technology can effectively replace bead-beating protocols for extraction of RNA. AFA extraction methods are more consistent, provide high quality RNA and are easy to perform.