Scientific Relevance

• ChIP-Seq is a powerful tool to map DNA binding proteins as well as histone modifications across the genome
• To allow the comparison of different studies and different input materials standardization of the sample preparation steps are required, which has been challenging
• NEXSON provides a simple but highly reproducible technique for efficient nuclei isolation, ensuring the generation of comparable chromatin maps from different sample types

Advantages of Adaptive Focused Acoustics® (AFA®)

• The high efficiency of nuclei isolation allows the scale down of input material to 10,000 cells/histone ChIP and 100,000 cells/transcription factor ChIP
• Increased robustness permits comparison across different tissues, cell types, disease stages, and laboratories
• The highly reproducible and efficient isolation of nuclei allows for novel high throughput derivates of ChIP technology