Scientific Relevance
- ChIP-Seq is a powerful tool to map DNA binding proteins as well as histone modifications across the genome
- To allow the comparison of different studies and different input materials standardization of the sample preparation steps are required, which has been challenging
- NEXSON provides a simple but highly reproducible technique for efficient nuclei isolation, ensuring the generation of comparable chromatin maps from different sample types
Advantages of Adaptive Focused Acoustics® (AFA®)
- The high efficiency of nuclei isolation allows the scale down of input material to 10,000 cells/histone ChIP and 100,000 cells/transcription factor ChIP
- Increased robustness permits comparison across different tissues, cell types, disease stages, and laboratories
- The highly reproducible and efficient isolation of nuclei allows for novel high throughput derivates of ChIP technology