Release of Microbial Cells from a Solid Matrix, Cell Lysis, and Shearing of Released Nucleic Acids Performed in a One-Step Procedure
This Application Note describes an Adaptive Focused Acoustics® (AFA®) procedure in which three of the above procedures are combined into one step – (1) the microbial cells are released from a solid matrix to which they are adhered, (2) the microbial cells are lysed, and (3) the DNA released from the cells is sheared to a desired fragment size with high precision and reproducibility. Samples may be processed in batches. The procedure is compatible with downstream automated purification and sequencing of the DNA fragments. It also has potential use for sample analysis involving lysis of cells of any type and detection of specific nucleic acid sequences using hybridization technology.