This technical note has been developed to streamline automated NGS library preparation by integrating DNA fragmentation with Covaris AFA™ technology into Eppendorf epMotionR 5075t/TMX library construction workflows, including Illumina® TruSeq® Nano DNA and TruSeq® DNA PCR-Free protocols. The Eppendorf line of epMotion automated liquid handling systems are designed to help researchers automate routine pipetting tasks to free up their time. Not only is the epMotion one of the most accurate pipetting stations on the market, by virtue of automation it helps to eliminate manual pipetting errors and maximizes the reproducibility of assay results. epMotion is available in four different formats and with different upgrade options, giving you the flexibility to tailor the system to your specific applications. The unique software makes programming easy and allows you to set-up even complex tasks with minimal training.