AFA-based Efficient DNA Extraction and Optional Mechanical Shearing from Mycobacteria smegmatis

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AFA-based Efficient DNA Extraction and Optional Mechanical Shearing from Mycobacteria smegmatis

We demonstrate efficient extraction and shearing of DNA from M. smegmatis prior to Next Generation Sequencing. Extraction of DNA from a strain of Mycobacterium smegmatis using the Covaris M220 Adaptive Focused Acoustics AFA instrument with a specific chemistry and set of energy conditions is shown to optimize DNA recovery.

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