Covaris DNA Shearing Guide for Illumina TruSight Tumor 170 NGS Assay
This document provides users with a complete set of protocols for all current Covaris Adaptive Focused Acoustics® (AFA®) instruments to use with the Illumina TruSight* Tumor 170 Kit. The following protocols have been developed to deliver the best outcome and compatibility for library preparation and subsequent sequencing applications. Covaris offers a full suite of AFA instruments to suit different levels of throughput, ranging from a single sample up to 96 samples in one run. The precise engineering of Covaris instruments and the specific design of consumables and holders allow tunable, defined delivery of adaptive focused acoustic energy to mechanically shear DNA. Illumina provides DNA shearing settings for Covaris E220evolution and LE220 Focused-ultrasonicators (Table 1) for the 8 microTUBE Strip V1 consumable. This application note provides settings for other Covaris instruments for DNA shearing to 90 to 250 bp in 55 μl of sample volume with the microTUBE-50. The microTUBE-50 has been specifically developed and optimized for 55 μl processing volume, and is the recommended consumable for this application.