Scientific Relevance
- A huge repertoire of non-coding transcripts is highly essential for proper gene regulation involved in many biological processes
- Long non-coding RNAs (lncRNAs) regulate chromatin states 2,3,4 and serve as landing platforms for chromatin modifying complexes
- ChIRP provides a powerful tool to map lncRNA binding sites on chromatin and can be combined with sequencing methods for a
genome-wide resolution
Advantages of Adaptive Focused Acoustics® (AFA®)
- AFA technology is a very gentle and tunable shearing method.
- Glutaraldehyde fixation time can be reduced allowing for shorter shearing times with better epitope preservation
- Big complexes bound to chromatin are more likely to be restored with all subunits
- lncRNAs with very few distinct binding sites are detected better because of good epitope preservations