Scientific Relevance

•  A huge repertoire of non-coding transcripts is highly essential for proper gene regulation involved in many biological processes
• Long non-coding RNAs (lncRNAs) regulate chromatin states 2,3,4 and serve as landing platforms for chromatin modifying complexes
•  ChIRP provides a powerful tool to map lncRNA binding sites on chromatin and can be combined with sequencing methods for a
  genome-wide resolution

Advantages of Adaptive Focused Acoustics^® (AFA^®)

• AFA technology is a very gentle and tunable shearing method.
• Glutaraldehyde fixation time can be reduced allowing for shorter shearing times with better epitope preservation
• Big complexes bound to chromatin are more likely to be restored with all subunits
• lncRNAs with very few distinct binding sites are detected better because of good epitope preservations