Pipetting Best Practices for Covaris 96 microTUBE Plate and 8 microTUBE Strip in Automated Liquid Handlers
Next-Generation Sequencing (NGS) platforms require controlled generation of DNA fragments to produce high-quality sequencing data. Covaris leverages its proprietary and patented Adaptive Focused Acoustics® (AFA®) technology to convert focused high frequency acoustic energy into mechanical force to randomly fragment nucleic acids in an isothermal, non-contact environment. For higher throughput settings, Covaris labware comes in several multi-well formats, including the 96 microTUBE Plate and 8 microTUBE Strip that can be mounted in 12 column SBS format racks. Hardware and methods are described here which maximize the robustness of robot pipetting in Covaris consumables, particularly strategies for piercing seals and recovering the maximum amount of sample after mechanical DNA shearing. Additionally, Covaris has developed the oneTUBE-10 product line compatible with the LE220-plus and ME220 Focused-ultrasonicators. The newly designed 96 oneTUBE-10 AFA Plate can be used as a typical SBS microplate and can be paired with the Automation Clamp for robust thin foil piercing.