Automated NGS Library Preparation with Beckman Biomek FXP and Covaris E220
Sequence capture, based on Roche Nimblegen technology, has been optimized though a collaboration with Roche1. The size of the DNA inserts are application- and project-specific and can range from 200 to 800 bp. For Sequence Capture projects, the sequencing effort is well calibrated to obtain the maximum of sequences for targeted regions. Two key parameters for high quality library preparation are the homogeneity of the library insert size and the un-biased fragmentation profile, which is why Biogemma chose to use Covaris AFA technology to fragment the DNA.