Affinity-based Enrichment Analyses of DNA Methylation MCIp, MeDIP, hMeDIP
Cytosine methylation/hydroxmethylation on DNA represent an important epigenetic regulatory layer that alters chromatin structure and influences transcription factor binding affinities
Such modifications are essential for proper gene expression states during development and often altered in diseases involving cancer development and metastasis
DNA methylation marks have huge potential as biomarkers 6 and display druggable targets
Precipitation-based methods provide a fast and easy tool to analyze genome-wide DNA methylation profiles and identify differentially methylated regions
Advantages of Adaptive Focused Acoustics® (AFA®)
Random fragmentation ensures unbiased representation of genomic regions:
Enables comparison of different samples e.g. tumour vs. healthy tissue or time course of follow-up samples AFA technology enables solubilization of RNA and DNA as well as chromatin from FFPE tissues and therefore allows for genome-wide DNA methylation profiling from FFPE tissue.