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In this study a method for automatic library preparation and size selection on a liquid handling robot is presented. The method utilizes selective precipitation of certain sizes of DNA molecules on to paramagnetic beads for cleanup and selection after standard enzymatic reactions.
This Application Note describes the use of Covaris Adaptive Focused Acoustics (AFA) technology to extract DNA from Formalin-fixed, paraffin-embedded (FFPE) tissues.
(Application Notes )
We observed linearity between read density and RNA input over the entire detection range and excellent agreement between replicates, but we observed significantly larger imprecision than expected under pure Poisson sampling errors. We use the control RNAs to directly measure reproducible protocol-dependent biases due to GC content and transcript length as well as stereotypic heterogeneity […]
Proctol: truXTRAC® FFPE total Nucleic Acid Plus Kit – Column Adaptive Focused Acoustics® (AFA)-based sequential RNA and DNA extraction from FFPE tissues using column-based purification Document Number: 010453
Covaris 96 microTUBE Plates (PN 010112)
(Product Inserts )
PRODUCT INSERT: Covaris 96 oneTUBE-10 AFA® Plate
(Product Inserts )
Protocol for: DNA Shearing with microTUBEs (<1.5kb fragments) S220/E220 Part Number 010305
Evaluation of Commercial DNA and RNA Extraction Methods for High-Throughput Sequencing of FFPE Samples
Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples Stine H. Kresse, et al
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Covaris provides tools and technologies to improve pre-analytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.