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Genome-wide oxidative bisulfite sequencing identifies sex-specific methylation differences in the human placenta
Gong, S., Johnson, M. D., Dopierala, J., Gaccioli, F., Sovio, U., Constância, M., . . . Charnock-Jones, D. S. (2018). Genome-wide oxidative bisulfite sequencing identifies sex-specific methylation differences in the human placenta. Epigenetics, 13(3), 228-239. doi:10.1080/15592294.2018.1429857
Protocol: AFA-Nanoemulsion™ : Rice Bran Oil in Water 2 mL Document Number: 010227
We investigated the use of Adaptive Focused Acoustics (AFA) technology to improve the performance of microtiter plate enzyme-linked immunosorbent assays (ELISAs). Experiments were performed with commercially available AFA instrumentation and off-the-shelf 96-well microtiter plate sandwich ELISAs.
Identification of Genes and Networks Driving Cardiovascular and Metabolic Phenotypes in a Mouse F2 Intercross
We have identified a large number of trait QTLs including loci driving variation in cardiac function on chromosomes 2 and 6 and a hotspot for adiposity, energy metabolism, and glucose traits on chromosome 8. Integration of adipose gene expression data identified a core set of genes that drive the chromosome 8 adiposity QTL. This chromosome […]
Protocol: DNA Shearing with LE220 Focused-ultrasonicator This Quick Guide provides DNA Shearing protocols when using microTUBE, microTUBE-50, microTUBE-15, microTUBE-500, or miniTUBE and a Covaris LE220 Focused-ultrasonicator. Document Number: 010156
The Covaris process, termed Adaptive Focused Acoustics (AFA) uses high frequency non contact ultra sonics derived from medical diagnostic ultrasound imaging and therapeutic lithotripsy applications, such as kidney stone disruption.
This Quick Guide provides DNA Shearing protocols when using microTUBE-130, microTUBE-50, microTUBE-15, microTUBE-500, or miniTUBE and a Covaris E220 Focused-ultrasonicator Document Number: 010308
Kuznetsov VI, Haws SA, Fox CA, Denu JM. General method for rapid purification of native chromatin fragments. J Biol Chem. 2018;
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