Many challenges are associated with sample preparations for proteomic applications, such as bottom-up analysis using LC-MS, Western Blot, and Reverse Protein Array (RRPA). Extracting proteins from FFPE required is commonly performed with complex and inefficient workflows.
Covaris and ProtiFITM have partnered to address challenges encountered when performing protein extraction from archived FFPE tissues. The partnership led to the development of a simple, efficient, and optimized proteomics workflow enabled by Covaris and ProtiFI. The Covaris Adaptive Focused Acoustics (AFA) technology combined with the Covaris truXTRAC FFPE Protein Extraction Kit drastically improves the sample prep by actively removing more paraffin while rehydrating tissue to isolate proteins prior to purification. ProtiFi, a protein purification technology, has developed S-TrapTMcolumns to trap AFA-released proteins and digest them prior to analysis.
Identify the true proteome with AFA paired with ProtiFi S-Trap
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Extract more proteins with 5% SDS, AFA, and S-Trap System for Mass Spectrometry Applications
Current Challenges for increasing protein recovery
Highly complex, inefficient, and cumbersome protein extraction workflows
Not standardized nor high-throughput workflows
Incomplete paraffin removal
Partial tissue rehydration and poor protein solubilization
Extraction and purification of low abundant proteins, such as membrane proteins
Utilization of toxic organic buffers
Combined Solution: Covaris AFA Paired with S-Trap Columns
Up to 97% protein recovery using S-Trap Column
Easy-to-use, simple, and efficient workflow
Rapidly process up to 96 samples in less than 5 hours
Highly improved active paraffin removal
Improved protein solubilization enabled by AFA and 5% SDS
No need for toxic organic solvents, such as xylene and ethanol
Interested in working with Covaris for your FFPE Protein Extractions?
Contact us for more information regarding working with Covaris on developing this protocol: