Scalable automation for NGS-grade DNA and RNA extraction from FFPE tissues
Automated active paraffin removal and simultaneous tissue rehydration
Up to 96 samples extracted in an SBS microplate format
4 hours total for 96 samples with minimal hands-on time
Tracking with 2D barcoded individual tubes or 8 strip
Easy to automate magnetic beads based purification
The truXTRAC automated workflow is a two-step process. DNA or RNA is extracted from FFPE tissues by AFA on a Covaris Focused-ultrasonicator. Then, DNA or RNA is purified from lysate with magnetic beads.
The entire workflow is fully scalable, from 12 samples per batch with benchtop instruments to 96 samples per batch with high throughput platforms.
Alternatively, purification can be automated on Perkin Elmer chemagen magnetic separation systems.
For more information, please contact Covaris at email@example.com
truXTRAC FFPE DNA 8 microTUBE Strip Kit – Bead Purification (96) (PN 520214): The kit contains reagents and consumables required for the high yield and high quality DNA extraction from 96 FFPE tissue samples, using a 8 microTUBE Strip and magnetic bead purification.
Note 3: truXTRAC 8 microTUBE Strip kit compatible only with E and LE-Series instruments and requires use of Cap Press Tool 8 microTUBE Strip V2 (PN 500469) and Heat Block Rack 12 Place 8 microTUBE Strip V2 (PN 500481)) to fit Covaris 8 microTUBE Strip into standard heating blocks
Improved FFPE DNA extraction for next generation sequencing using adaptive focused acoustics technology Ling Lin, et al ; 2014. “We found that Covaris had superior performance overall: (1) DNA yields were 6 times higher, and less specimens failed extraction: this is critically important when dealing with small tumor biopsies. (2) DNA was more readily available for PCR amplification. (3) Mean coverage of DNA fragments with high GC content was higher and more reproducible. (4) Library complexity was higher. In addition, the Covaris protocol required less hands-on time in the lab, and eliminated the need for hazardous organic solvents for paraffin removal.”
Genomic DNA Extraction Methods Using FFPE Tissue K Potluri, A Mahas, MN Kent, S Naik, M Markey – Analytical Biochemistry, 2015 “Of the three extraction methods, AFA samples showed increased amplicon length and decreased PCR failure rate. These findings support AFA as an improvement over previous DNA extraction methods for FFPE tissues.”
DNA Extraction of Lung Cancer Samples for Advanced Diagnostic Testing
Jeffrey Conroy et al, 2015 “FFPE tumor samples prepared using the Covaris truXTRAC isolation kit provides an efficient system for generating high quality DNA samples even from lung cancer specimens that previously failed testing with QIAGEN extraction. [..]. Additionally, DNA requirements for NGS based testing can be reduced 25-50% without loss of assay performance or analytic sensitivity as validated in our CLIA laboratory.”