The tissueTUBE is a unique biomedical device constructed of strong, flexible plastic. When used in combination with an AFA tube, tissueTUBE devices facilitate the closed vessel cryofracture of a biological tissue sample in a convenient, controlled, and easy-to-use manner. The film used in the tissueTUBE is made of Kapton, a “space-age” polymer originally developed to withstand the extreme temperature fluxes experienced in orbit. The tissueTUBE can be used with any of our cryoPREP Dry Impactors® and allows you to extract target molecules in four easy steps: Load tissue in tissueTUBE, Snap (flash-freeze), Crack (dry pulverize), Pop (AFA-enabled cavitation to actively extract).
Advanced Tissue Processing Workflows with cryoPREP
Insert: Place the tissue in the center of the tissueTUBE
Snap Freeze:Flash freeze the sample in liquid nitrogen to thermally stabilize biomolecules. Samples may be stored at -80C.
Crack with cryoPREP: Dry pulverize the sample to increase surface area and disrupt extracellular matrix without the need for use of organic solvents. Samples may be stored at -80C.
Pop with AFA: Acoustically extract biomolecule in an appropriate stabilizing buffer prior to purification or analysis.
Tissue device consumables and accessories required to process low and hard tissue masses in the range of 1-10 mg using the t-PREP Extraction System.