The t-PREP is designed for biomarker extraction from small (< 10 mg) tissue samples. The integration of the entire homogenization process from flash freezing at the point-of-collection to biomolecule extraction makes the t-PREP an ideal tool for extracting nucleic acids, chromatin, proteins, and small molecules from precious tissues. The t-PREP workflow increases throughput by decreasing processing time to a few seconds per sample and improves the extraction efficiency when coupled with AFA®.
Figure 1. Improved protein recovery and sample complexity from mouse liver tissue processed in the t-PREP with AFA versus Dounce homogenizer. Representative image of a Coomassie-stained 2DGE.
Features & Benefits
Designed for < 10 mg
Highest recoveries from small tissue samples (e.g., biopsies)
Figure 1: High yield and reproducible protein extraction from small tissue samples
Total protein was extracted in 270 µl Buffer B from ~15 mg of mouse heart muscle using either the t-PREP or a Dounce homogenizer. Mean protein yield from the 4 t-PREP samples is 181 +/- 3 µg protein/ mg tissue (CV = 1.7%) compared to 68 +/- 9 ug protein / mg tissue with the Dounce homogenized tissue.
Figure 2: Improved protein extraction and sample complexity
Improved protein recovery and sample complexity from murine liver tissue processed in the t-PREP with AFA versus Dounce homogenizer. Representative Coomassie stained 2DGE; enlarged area highlights 4 proteins present in the t-PREP processed samples significantly higher levels compared to Dounce processed samples (e.g., circled spots).
Figure 3: Improved preservation of phosphorylated proteins
Improved preservation of phosphorylated proteins in mouse brain tissue processed in the t-PREP with AFA versus Dounce homogenizer. Representative ProQ Diamond (phosphorylated proteins) and SYPRO Ruby (Total Proteins) stained 2DGE. The t-PREP processed samples demonstrate a higher level of phosphorylated proteins overall; 5 specific spots are indicated where staining is significantly higher in the phosphorylated protein stain, while the total protein stain from the t-PREP is still comparable with the Dounce processed samples.
Covaris provides tools and technologies to improve pre-analytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.