High-efficiency biomarker extraction from dried blood samples using Adaptive Focused Acoustics® (AFA™)

Poster presented at ASHG2015 – Here, we present two innovations that together overcome many of the drawbacks of the current DBS technology: (1) a novel sampling device for collecting capillary blood (truCOLLECT™) that provides integrated sample barcoding, eliminates the need for a drying period before shipment, and yields DNA and protein amounts similar to those seen with fresh blood; and (2) use of Adaptive Focused Acoustics (AFA™) for efficient release of biomarker from truCOLLECT™ devices as well as from conventional DBS (truXTRAC™ DBS). The AFA™-based extraction process is highly automated and allows batch processing with less than 30 min hands-on-time per 96 samples.