Mapping the epigenetic and transcription factor landscape is essential for understanding gene regulatory mechanisms and holds huge potential for identification of biomarkers and targets for epigenetic therapy.
However, existing ChIP workflows are largely labor-intensive and require time-consuming optimizations for the cell or tissue type of interest. During this webinar, a fast, easy, and scalable workflow that could be applied to any input material of choice will be reviewed.
Specifically, the speaker will discuss optimized cell lysis and nuclei isolation prior to chromatin shearing and IP steps via ultrasonification followed by sucrose centrifugation. This method provides efficient and reproducible chromatin preparation and enables performance of ChIP assays within two days from virtually any mammalian cell type, including challenging primary material.
You will learn about:
- Optimized cell lysis and nuclei isolation prior to chromatin shearing
- A streamlined and easy workflow for ChIP from virtually any mammalian cell type
- A reliable two-day ChIP workflow from primary cells