Join us for our CoLab presentation!
Topic: Standardization of the ChIP workflow using Covaris Adaptive Focused Acoustics (AFA) to study the transcriptional regulation of SOD1 in ALS pathogenesis
Date: 17 October 2018
Time: 10:30 to 11:00 a.m.
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder that causes gradual degeneration and elimination of neurons that control voluntary muscles. Certain familial ALS is linked to mutations in copper, zinc superoxide dismutase 1 (SOD1, cytosolic SOD). This antioxidant enzyme destroys free superoxide radicals in the cytosol, however, mutant forms are hypothesize to be defective.
Wildtype (WT) SOD1 may acquire an altered toxic profile and contribute to the vast majority of sporadic ALS cases. To date, therapeutically relevant mechanisms on how mutant and WT SOD1 cause neurodegeneration remains unknown.
One possible mechanism of disease progression is thought to be through certain epigenetic modifications in neurons, such as SOD1 binding events. To evaluate the role of SOD1 in neurons, Byung Woo Kim, a fifth year PhD candidate from the Division of Neuropathology at The Johns Hopkins School of Medicine will present his findings from the optimized ChIP workflow using truChIP coupled with the Covaris acoustic shearing technology. Moreover, he will discuss the pairing of Covaris with a robust immunoprecipitation (IP) protocol that significantly reduced the variability observed with other traditional bead-based workflows.
Clinically relevant, these findings suggest a nuclear function of SOD1 and the potential role aberrant nuclear human SOD1 (hSOD1) plays in the degeneration of vulnerable neurons, which provides a possible mechanism of ALS pathogenesis.
The 68th Annual Meeting of the American Society of Human Genetics ( ASHG ) will be held at the San Diego Convention Center in San Diego, California, from Tuesday, October 16 through Saturday, October 20. The ASHG Annual Meeting is the largest human genetics meeting and exposition in the world. This year’s meeting is expected to attract over 8,500 scientific attendees, plus almost 460 exhibiting companies. The meeting provides a forum for the presentation and discussion of cutting-edge science in all areas of human genetics.
The American Society of Human Genetics (ASHG) is the primary professional membership organization for human genetics specialists worldwide. ASHG members and leading scientists from around the world are selected to present their research findings at invited, platform, and poster sessions. Abstracts presented at the Annual Meeting are published online and are citable. ASHG’s Annual Meeting also features a trade show floor that offers attendees the opportunity to view state-of-the-art medical and laboratory equipment, products, services, and computer software designed to enhance human genetics research, teaching, and consultation.
Make the most of your time at the ASHG Annual Meeting this year by sharpening your networking skills just before the meeting begins! In this session, instructors will provide an overview of networking, describe its importance, and help you make the most of opportunities to meet scientific professionals throughout the week. The session will feature interactive exercises, where you can practice your networking skills with peers, instructional leaders, and scientific professionals attending the meeting.