Pairing Covaris truChIP® and AFA® with the Chromatrap® Bead-free Immunoprecipitation Technology

Covaris and Chromatrap have partnered to offer a highly scalable and simple ChIP workflow enabling researchers to easily isolate protein-DNA complexes for downstream applications, such as next-generation sequencing (NGS), ChIP-qPCR, and Mass Spectrometry (MS). Pairing these two workflows provides unmatched flexibility, speed, broad capability for a wide dynamic range of inputs, and automatable protocols. This spin column-based workflow eliminates many of the challenges encountered when working with magnetic beads. A typical bead-based workflow takes around 10 hours with over 10 wash steps and incubations. This rapid centrifugation-based workflow requires only three simple washes using individual spin columns or 96-well plates and can be completed in a single day. Using the Covaris Adaptive Focused Acoustics® (AFA®) technology, researchers can easily isolate and reproducibly shear soluble chromatin without disrupting the protein-DNA interactions for immunoprecipitation (IP) with Chromatrap’s bead-free Protein A and G ChIP kits.

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