Utilization of the Covaris® 96 oneTUBE-10 AFA Plate Streamlines NGS Sample Prep

The most commonly used approach for fragmenting genomic material utilizes mechanical shearing. This method is advantageous in that it introduces little to no bias as a result of processing. However, mechanical shearing methods necessitate the use of specialized equipment and consumables and, currently, require several manual transfer steps for processing. The workflow presented here eliminates the need for additional transfer steps, reducing the possibility for sample loss and decreased process yield. Additionally, this workflow addresses many of the inconveniences currently associated with gDNA fragmentation on Covaris Focused-ultrasonicators.