In this case study, DNA shearing in the oneTUBE was paired with Swift Biosciences™ Accel-NGS 2S® Kit for NGS library construction. The 2S kit preserves complexity from low input samples due to enhanced end repair chemistry for highly efficient adapter ligation in a single tube workflow. This combination enables for the first time DNA shearing, end repair, and adapter ligation in the same vessel, thereby eliminating cumbersome sample transfers. We present sequencing data from libraries constructed with this workflow and compare them to libraries constructed according to standard “with transfer” protocol in microTUBE.
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