Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.

Process Development Seminar

Carl Beckett VP of Business Development will be discussing how AFA can control nucleation and crystal distribution on June 21 at the Cambridge R&D Process Development Seminar

Carl Beckett VP of Business Development will be discussing how AFA can control nucleation and crystal distribution on June 21 at the Cambridge R&D Process Development Seminar.

R&D Process Development Seminar
Symposium focusing on R&D Process Development

June 21 @ 8:00 am5:00 pm in Cambridge, MA

Evaluation of Next Generation Library Preparation using DNA Extracted from Dried Blood Spots using truXTRAC® DBS DNA kit

Dried Blood Spots (DBS) provide an easy and inexpensive way to collect and store peripheral blood specimens from infants, children nd adults.[1] The use of DBS allows for less invasive procedures for patients and easier shipment while still providing the ability to run molecular or clinical biochemical assays. This convenient method for the long term room-temperature storage of materials also minimizes storage and archival space. The truXTRAC DBS DNA kit is designed for controlled and efficient extraction of next-generation sequencing (NGS)-grade DNA from DBS samples using Adaptive Focused Acoustics® (AFA®). Covaris AFA enables sample rehydration while providing simultaneous cell lysis and controlled mechanical DNA shearing, resulting in high-yield, high-quality and NGS library preparation ready DNA.

Adaptive Focused Acoustics Processing to Aid Recovery of Fungi from CF Patient Sputum Samples

Background: Cystic fibrosis (CF) is a genetic disorder that afflicts over 70,000 people worldwide. It is characterized by viscous mucus that accumulates in the lungs creating challenges for microbiology labs processing sputa toisolates lower growing fungal species. Our laboratory attempted to liquefy and homogenize mucoid sputum samples by exposure to Adaptive Focused Acoustics™ (AFA) in order to increase recovery of fungal pathogens.

H.B. Miller, et al