In this case study, DNA shearing in the oneTUBE was paired with Swift Biosciences™ Accel-NGS 2S® Kit for NGS library construction. The 2S kit preserves complexity from low input samples due to enhanced end repair chemistry for highly efficient adapter ligation in a single tube workflow. This combination enables for the first time DNA shearing, end repair, and adapter ligation in the same vessel, thereby eliminating cumbersome sample transfers. We present sequencing data from libraries constructed with this workflow and compare them to libraries constructed according to standard “with transfer” protocol in microTUBE.
Woburn, MA – November 14, 2017 – Today, Covaris Inc. announced the release of the 96 oneTUBE-10 AFA Plate, which enables scalable and simplified workflows by leveraging Covaris’ gold standard focused-acoustic technology to meet rapidly increasing Next-Generation Sequencing (NGS) demands for high-throughput and low-input library preparation. By using the oneTUBE-10 offering, transfer steps within the NGS library prep workflow have been eliminated from mechanical DNA shearing (using Covaris’ Adaptive Focused Acoustics® (AFA®) technology) through end repair, A-tailing, ligation, and SPRI clean-up. The oneTUBE-10 product is specifically designed for use with Covaris’ AFA technology for DNA shearing at volumes ranging from 10 to 50 µL, achieving highly accurate and reproducible results.
This new AFA vessel, is made of an engineered polymer for low impedance and efficient transmission of acoustic energy. oneTUBE-10 effectively controls acoustic cavitation to enable reproducible and precisely-tuned hydrodynamic shear forces. Designed to SLAS standards, oneTUBE-10 is compatible with standard heat blocks, magnets, and thermocyclers. oneTUBE-10 can be used on any liquid handling platform, providing a universal solution for streamlining NGS workflows. Paired with the LE220-plus (Covaris’ newest Focused-ultrasonicator), NGS workflows are easily standardized by integration with high-throughput laboratory automation.
Covaris Founder and CEO, Jim Laugharn, said, “oneTUBE-10 is a game-changer for NGS workflows. The combination of the Covaris AFA-energetics, the process behind the industry gold standard for robust, random, and unbiased DNA shearing, with a uniquely engineered polymer consumable enables highly automated library preparation, nucleic acid extraction, and additional applications in a single tube. The improved workflow possibilities are unlimited!”
Future applications of oneTUBE-10 include FFPE extraction, enhanced ligation enzymatic reactions, and other biomolecular processes utilizing the Covaris technology. AFA can now be applied at various steps including bead binding, mixing, and elution, while scaling down to low volumes. Additionally, oneTUBE-10 product iterations will include an 8-strip format and will scale up to 384 well formats and beyond.
About Covaris, Inc.
Covaris is a recognized industry innovator in preanalytical sample preparation workflows involving sample stabilization, extraction, and processing. Leveraging its patented Adaptive Focused Acoustics (AFA) technology and novel engineering solutions, Covaris has developed comprehensive packages of instrumentation, applications, reagents, and consumables to address the increasing sensitivity requirements of analytical technologies such as NGS and mass spectrometry. Covaris Focused-ultrasonicators are recommended by all major NGS platform providers, and are used by leading Genome Centers worldwide. The Covaris AFA process is extensively cited in peer-reviewed research articles on DNA fragmentation, ChIP, FFPE nucleic acid extraction, metabolomics, small molecule extraction, proteomics, and nanoemulsion formulation. For more information, please visit: www.covaris.com.
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This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.
Covaris provides tools and technologies to improve pre-analytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.