Pipetting Best Practices for Covaris 96 microTUBE Plate and 8 microTUBE Strip in Automated Liquid Handlers

Next-Generation Sequencing (NGS) platforms require controlled generation of DNA fragments to produce high-quality sequencing data.
Covaris leverages its proprietary and patented Adaptive Focused Acoustics® (AFA®) technology to convert focused high-frequency acoustic
energy into mechanical force to randomly fragment nucleic acids in an isothermal, non-contact environment. For higher throughput settings,
Covaris labware comes in several multi-well formats, including the 96 microTUBE Plate and 8 microTUBE Strip that can be mounted in
12 column SBS format racks. Hardware and methods are described here which maximize the robustness of robot pipetting in Covaris
consumables, particularly strategies for piercing seals and recovering the maximum amount of sample after mechanical DNA shearing.

Magnetic Bead-based Clean-up Using AFA-energetics™

DNA shearing is the first step in NGS library prep workflow and AFA-based DNA shearing from Covaris is the industry gold standard.
In order to support downstream NGS library prep applications, Covaris protocols support contact-free micromixing during magnetic beadbased
clean-up steps. Here, we show how Adaptive Focused Acoustics® (AFA®) technology can replace conventional pipetting to mix a
sample, specifically during a SPRI* clean-up step, in the new, automatable 96 oneTUBE-10 AFA plate.

Affinity-based Enrichment Analyses of DNA Methylation MCIp, MeDIP, hMeDIP

One Pager: Affinity-based Enrichment Analyses of DNA Methylation MCIp, MeDIP, hMeDIP

Document Number: M020091