Nucleic Acid Extraction from Laser-Capture Microdissected (LCM) FFPE Tissues

Nucleic Acid Extraction from Laser-Capture Microdissected (LCM) FFPE Tissues

Scientific Relevance

  • Formalin-fixed, paraffin-embedded (FFPE) tissue is a widely used method for long-term clinical sample storage, but it comes with significant drawbacks due to the harsh nature of this method
  • Researchers are increasingly realizing the importance of investigating subpopulations of cells within homogeneous tissue and the cross-talk between these cells, especially for tumor cells and their surrounding stroma
  • Laser-Capture Microdissection (LCM) can be used on FFPE tissues and enables researchers to target and collect subsets of cells for DNA and RNA analysis by examining the tissue structure through microscopy

Advantages of Adaptive Focused Acoustics® (AFA®)

  • Efficient extraction of high-quality nucleic acids from FFPE tissues that can be used in PCR, qPCR, and NGS
  • Reduced QNS rates with even the smallest inputs
  • Longer RNA fragments with high DV200 scores, enabling detection of rare fusion events
  • Simultaneous RNA and DNA extraction from the
    same FFPE tissue

Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo

Chromatin immunoprecipitation coupled with next-generation sequencing technology (ChIP-seq) has enabled investigators to generate high-resolution maps of genomic binding sites for transcription factors and other members of the chromatin-associated machinery.

AFA-energetics™-Mediated RNA Extraction from Yeast

This application note describes the optimized AFA-energetics dose to lyse and extract RNA from S. cerevisiae. We provide guidelines for
a sample preparation protocol using the Covaris LE220-plus Focused-ultrasonicator and 96 oneTUBE-10 AFA Plate that can be used by
investigators to lyse S. cerevisiae and extract RNA, along with supporting data.