Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo

Chromatin immunoprecipitation coupled with next-generation sequencing technology (ChIP-seq) has enabled investigators to generate high-resolution maps of genomic binding sites for transcription factors and other members of the chromatin-associated machinery.

AFA-energetics™-Mediated RNA Extraction from Yeast

This application note describes the optimized AFA-energetics dose to lyse and extract RNA from S. cerevisiae. We provide guidelines for
a sample preparation protocol using the Covaris LE220-plus Focused-ultrasonicator and 96 oneTUBE-10 AFA Plate that can be used by
investigators to lyse S. cerevisiae and extract RNA, along with supporting data.

Pipetting Best Practices for Covaris 96 microTUBE Plate and 8 microTUBE Strip in Automated Liquid Handlers

Next-Generation Sequencing (NGS) platforms require controlled generation of DNA fragments to produce high-quality sequencing data.
Covaris leverages its proprietary and patented Adaptive Focused Acoustics® (AFA®) technology to convert focused high-frequency acoustic
energy into mechanical force to randomly fragment nucleic acids in an isothermal, non-contact environment. For higher throughput settings,
Covaris labware comes in several multi-well formats, including the 96 microTUBE Plate and 8 microTUBE Strip that can be mounted in
12 column SBS format racks. Hardware and methods are described here which maximize the robustness of robot pipetting in Covaris
consumables, particularly strategies for piercing seals and recovering the maximum amount of sample after mechanical DNA shearing.