Simplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications
Abstract: Large-scale analysis of histone marks and transcription factor interactions requires high-quality chromatin, use of validated Chromatin Immunoprecipitation (ChIP)-grade antibodies, and optimized reagents to produce meaningful and reproducible results. In this application note, we demonstrate:
i. Isolation of nuclei using Covaris Adaptive Focused Acoustics® (AFA®) technology followed by purification using a sucrose ultracentrifugation step standardizes ChIP workflows and enables scientists to process-hard-to-lyse samples and reduce input requirements, which is frequently encountered when working with clinical samples.
ii. Pairing Covaris truChIP Kits with Chromatrap bead-free immunoprecipitation (IP) technology provides a simple, sensitive, and scalable workflow for downstream applications, such as ChIP-qPCR and ChIP-seq.