Affinity-based Enrichment Analyses of DNA Methylation MCIp, MeDIP, hMeDIP

Cytosine methylation/hydroxmethylation on DNA represents an important epigenetic regulatory layer that alters chromatin structure and influences transcription factor binding affinities.  These modifications are essential for proper gene expression states during development and are often altered in diseases involving cancer development and metastasis.

DNA methylation marks have huge potential as oncology biomarkers and display druggable targets.  Precipitation-based methods provide a fast and easy tool to analyze genome-wide DNA methylation profiles and identify differentially methylated regions

DNA methylation mark detection has a variety of challenges associated with it.  For example, affinity-based enrichment methods display an inherent sequence bias and therefore require a way to perform unbiased and reproducible DNA shearing for optimal resolution and coverage.  Also, sequences of varying fragment length will precipitate with different efficiency and therefore require tight DNA fragment size distributions.

Do you encounter some of these challenges in your research? Did you know that Covaris Adaptive Focused Acoustics® (AFA®) technology can help resolve these challenges? Covaris AFA-enabled random fragmentation ensures unbiased representation of genomic regions.  Additionally, AFA enables comparison of different samples (e.g. tumor vs. healthy tissue) or time course of follow-up samples.  Covaris can enable solubilization of RNA and DNA as well as chromatin from FFPE tissues, which allows for genome-wide DNA methylation profiling from FFPE tissue.

Are you interested in working with Covaris for your DNA Methylation MClp, MeDIP, hMeDIP research? Contact applicationsupport@covaris.com today!

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