Biology at the Speed of Sound™
Adaptive Focused Acoustics® (AFA) is an advanced patented acoustic technology enabling the non-contact and isothermal mechanical processing of samples with AFA-energetics™ enabled Covaris Focused-ultrasonicators. AFA-energetics™ utilizes advanced medical grade acoustic transducers to generate highly controlled and discrete bursts of focused high-frequency acoustic energy for an efficient and reproducible sample processing. The very high-frequency ultrasound utilized in AFA results in a wavelength of only a few millimeters, enabling the acoustic energy to be focused into a discrete zone within a sample vessel. This focused and efficient delivery requires a minimal amount of energy input avoiding the adverse effects of excess energy such as damaging heat, experimental variability, and sample over-processing of traditional typical sonicators.
Biology at the Speed of Sound™ means you are using the patented Covaris AFA technology to accelerate your research starting with single tube processing all the way through 96 sample and beyond high-throughput robotic integration enabled workflows. The speed of sound in water is 1500 meters per second, or very fast. Covaris AFA will uncover the true biology of your sample at the speed of sound saving time, resources, and precious biological material. At Covaris our focus is to help you harness the full potential of your precious sample.
Bring AFA-energetics to your samples- The Covaris Grant Program
Facing the need to truly scale up your sample preparation workflow? Reduce time around time while increasing reproducibility?
Our Grant Program supports outstanding research projects that advance scientific discovery through the improvement in preanalytical sample preparation for clinical samples.
Contact us with your challenges! Submit your grant application to Bill Campbell, VP of Marketing, firstname.lastname@example.org
Many of the pre-analytical tools used today in biological research are based on passive diffusion processes and other antiquated methodologies, placing significant limitations on the speed, efficiency, reproducibility, and throughput of these tools. This is especially significant in the rapidly advancing analytical techniques of medical oncology; clinical outcomes may be limited by out of date pre-analytical tools.
Covaris AFA-energetics enables the standardization of these processes and the development of highly scalable workflows. See below of list challenges for which AFA-energetics has been applied.
1. FFPE tissues biomarkers extraction
|The passive diffusion-based organic solvent removal of paraffin is cumbersome, time-consuming, inefficient and an unnecessary health risk to the lab personnel.||Paraffin is actively and efficiently removed from the FFPE tissue without the use of organic solvents enabling high-yield and quality extraction of nucleic acids or proteins, and standardization of FFPE sample preparation for NGS based applications.|
2. cfDNA extraction and purification
|The low prevalence of circulating tumor DNA (ctDNA) in a high background of native cfDNA has proven challenging to extract with the passive-diffusion based extraction/purification methodologies.||Active-extraction of cfDNA from histones and chaperone proteins has enabled higher cfDNA yield and NGS library complexity reflecting the true physiological profile of cfDNA.|
3. Magnetic binding of nucleic acids
|The mechanism for nucleic acid binding to magnetic beads relies on passive diffusion which significantly affects yield, throughput and reproducibility.||Active bead binding has enabled the possibility of standardization by reducing the binding time and maximizing nucleic acid binding to magnetic beads.|
4. Magnetic bead washing
|Washing of magnetic beads using passive diffusion is inefficient and leads to adoption of multiple wash steps and utilization of less stringent wash conditions leading to sample loss.||Covaris AFA active acoustic mediated bead washing can be carried out more rapidly and using higher stringency wash solutions to prevent sample loss.|
|Ligation reactions of fragments with blunt-ends or T/A overhangs are typically performed at room temperature, where the ligase is active, but are limited in product yield because molecular movement prevents the formation of a quaternary complex.||AFA-induced micromixing counters the reduced diﬀusion in buﬀers with crowding agents, increases efficiency and truly reduces incubation times necessary to achieve high yields.|
6. Nucleic acid elution from magnetic beads
|Elution of precious nucleic acids from magnetic beads using passive diffusion-based elution methods is inefficient and leaves a significant amount of nucleic acids behind.||Active nucleic acid elution has enabled standardization by reducing the elution time and increasing the yield of eluted DNA from magnetic beads.|
7. DNA shearing of clinical samples
|Preparation of NGS libraries with DNA digested with difficult to control enzymes or antiquated traditional sonicator technologies from the last century don’t work with limited input and variable clinical samples.||Mechanical DNA Shearing is considered the gold-standard for fragmenting of DNA for clinical NGS by enabling highly reproducible, automatable, and tight fragment distribution shearing of DNA.|
8. Hybridization for enrichment workflows
|Passive diffusion for enrichment of libraries for whole exome sequencing and targeted panels has become a limiting factor for their clinical utility due to the more complicated workflow and longer turnaround time (TAT).||With active hybridization, we have reduced the hybridization time to 15 minutes or less without sacrificing data quality. It enables the preparation of NGS library and enrichment in less than a work day, significantly reducing the TAT for clinical samples.|
9. Chromatin shearing for epigenomics
|Biased fragmentation and damage to protein epitopes reduce resolution and sensitivity.||AFA has enabled the efficient and reproducible isothermal shearing of chromatin without damaging the epitopes. This has led to a significant increase in ChIP sensitivity and orders of magnitude reduction in the number of cells required for ChIP, enabling ChIP as a clinical tool.|
Covaris provides tools and technologies to improve pre-analytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.