Pre-analytical processes are increasingly recognized as the limiting factors for sensitivity and specificity of biomarker detection. This is especially relevant for highly advanced pre-analytical processes and analytical methods such as next-generation sequencing or mass spectrometry.
With AFA®, Covaris offers complete solutions, including instrumentation, reagents, consumables, and protocols that address all pre-analytical processes and sample prep challenges.
thawing and sample transfers during extraction lead to temperature sensitive biomarker loss and degradation
cryoPREP® and t-PREP® consolidate collection, rapid stabilization, dry cryofracture, and stabilized extraction into a single vessel and enable strict temperature control during extraction
truCOLLECT® Kit enables the collection of a predefined sample volume (maximum), desiccation and/or chemical sample stabilization, transportation, storage, and eventual efficient AFA extraction for biomarkers
Incomplete de-paraffinization by harsh chemicals reduces biomarker yield and quality
truXTRAC® FFPE Kit allows highly effective de-paraffinization without harsh chemicals, efficient rehydration, and non-contact tissue lysis
Dried Blood Spots
Poor release from filter paper reduces biomarker yield
truXTRAC® DBS Kit enables rapid, iso-thermal, high biomarker yields from single 3-mm punch
Robust cell walls require tedious workflows and reduce biomarker yield
Lack of Standardization: Outdated Passive extraction methodologies with low yield and reproducibility, and complex protocols not amenable to automation prevent efficient standardization of the workflow.
truXTRAC cfDNA is an AFA mediated active extraction and purification kit with high yield, high reproducibility ambient temperature protocol designed with standardization and high throughput processing in mind.
Pipetting viscous mucous from infected patients’ (i.e. Cystic Fibrosis) sputum is difficult and therefore leads to missed species identifications from a heterogeneous sample population
The Covaris AFA-based sample prep protocol disrupts biofilms without causing damage to fungi, yeast, and bacterial cells enabling sample pipetting for routine culture and processing on flow-through instrumentation
DNA or RNA shearing
Biased fragmentation or wide distributions of fragment sizes reduce library complexity and yield
truSHEAR™ provides unbiased fragmentation and highly reproducible, tight distribution of fragment sizes for Next Generation Sequencing and other advanced genomic analysis
Chromatin shearing for ChIP
Biased fragmentation and damage to protein epitopes reduce resolution and sensitivity