Sisi Li, PhD Candidate, Investigates How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding in the Brain

In this Covaris sponsored webinar, which LabRoots will host May 3rd, participants will learn how 5hmC may regulate gene expression by mediating transcription factor binding in vivo.

While stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how it induces alterations in brain chemistry leading to mental illness remains poorly understood. In this webinar, the speaker will present her research findings, which focused on the novel environmentally-sensitive DNA methylation mark, 5-hydryoxymethycytosine (5hmC) which is highly enriched in the brain, sensitive to stress, and associated with active neuronal transcription.

Utilizing an optimized protocol for chromatin immunoprecipitation (ChIP) from a limited amount of brain tissue, Sisi Li generated data confirming that exposure to stress resulted in a significant decrease in the binding of a transcription factor to a candidate gene. In addition to ChIP data, Li will also present RNA-Seq and EMSA data, and describe how to prepare chromatin from low amounts of frozen brain tissue for ChIP applications.
Li will emphasize the importance of optimizing the formaldehyde fixation step of ChIP, and provide insightful tips on how to minimize tissue loss during ChIP sample preparation.