Active paraffin removal with truXTRAC FFPE DNA, enabled by Adaptive Focused Acoustics (AFA), promotes complete sample rehydration, thus improving tissue digestion by proteinase K and enhancing the dissociation of biomolecules for improved extraction of DNA. The entire process is performed in a single tube without transfer steps and last 5 minutes.
High quality and yield of DNA using AFA technology
Simplified workflow with minimal hands-on time
Greater library complexity
Unbiased DNA extraction
Longer fragment length
Improved DNA Amplifiability as measured by Illumina’s QC Kit
Higher Coverage Depth and Uniformity Compared to Competing Extraction Kits
Next Generation Sequencing applications require precisely controlled DNA fragmentation as part of the library preparation. Covaris AFA is the method of choice for DNA fragmentation in Genome Centers worldwide, because the DNA fragment size is easily controlled and highly reproducible. The DNA shearing step can be integrated into the truXTRAC workflow, greatly simplifying the library preparation protocol from FFPE tissue samples.
Superior Paraffin Removal with Covaris AFA as Compared to Solvent-based Paraffin Removal
truXTRAC FFPE DNA microTUBE Kit -Column Purification (25)
The kit contains reagents and consumables required for the high yield and high quality DNA extraction and purification from 25 FFPE tissue samples. Optimized to work with FFPE sections ≤ 25 μm containing ≤5 mg of tissue.
Centrifuge and Heat block microTUBE Adapters (quantity 10, PN 500406): Heat Block microTUBE Adapters are designed to fit a microTUBE into a heating block designed for 1.5 or 2 mL microcentrifuge tubes. It is required for Proteinase K incubation and to reverse formaldehyde crosslinks.