Figure 1. RNA was extracted from FFPE tissue samples using Covaris truXTRAC and a traditional FFPE extraction kit, which uses organic solvents to remove paraffin. RNA fragment lengths were determined on a fragment analyzer and DV200 scores (the ratio of RNA longer than 200 base pairs) were calculated.
Higher Nucleic Acid Yields
• Reduce failure rates for precious samples prior to NGS
• Increase library complexity
• Improve sequencing coverage
Superior DNA Yields with AFA-energetics
Figure 2. DNA was extracted from FFPE tissue samples using Covaris truXTRAC and a traditional FFPE extraction kit, which uses organic solvents to remove paraffin. DNA yields were determined by Qubit.
Optimized to work with larger FFPE inputs: ≤ 45 μm of FFPE sections. Kit contains reagents and consumables required for extraction and purification of DNA and RNA from 25 FFPE tissue
Centrifuge and Heat block microTUBE Adapters (quantity 10, PN 500406): Heat Block microTUBE Adapters are designed to fit a microTUBE into a heating block designed for 1.5 or 2 mL microcentrifuge tubes. It is required for Proteinase K incubation and to reverse formaldehyde crosslinks.