Products and Services
truXTRAC® FFPE DNA
NGS-quality DNA from FFPE tissue samples
- Ideal for low sample inputs and difficult-to-extract FFPE samples
- High quality, high yield of DNA from FFPE tissue
- Simple, easy-to-use workflow with minimal hands-on time
- Hands-free, active deparaffinization without organic solvents
Ordering Information
| Product Name | Part Number | Samples/Kit | Price | |
| truXTRAC FFPE DNA microTUBE Kit – Column Purification | 520136 | 25 | $306.00 |
Workflow
More Information
Features & Benefits
NGS-grade RNA and DNA from FFPE tissues
Active paraffin removal with truXTRAC FFPE DNA, enabled by Adaptive Focused Acoustics (AFA), promotes complete sample rehydration, thus improving tissue digestion by proteinase K and enhancing the dissociation of biomolecules for improved extraction of DNA. The entire process is performed in a single tube without transfer steps and last 5 minutes.
- High quality and yield of DNA using AFA technology
- Simplified workflow with minimal hands-on time
- Greater library complexity
- Unbiased DNA extraction
- Longer fragment length
Whole genome sequencing results from Covaris extracted FFPE DNA generates greater coverage depth across the genome with coverage uniformity similar to DNA extracted from fresh-frozen tissues. With Covaris truXTRAC coding and non-coding DNA regions are extracted with the same efficiency, delivering unbiased library preparation. A comparison of active and passive FFPE DNA extraction methods and effects on downstream analysis metrics (pdf).
Direct integration with NGS library preparation methods
Next Generation Sequencing applications require precisely controlled DNA fragmentation as part of the library preparation. Covaris AFA is the method of choice for DNA fragmentation in Genome Centers worldwide, because the DNA fragment size is easily controlled and highly reproducible. The DNA shearing step can be integrated into the truXTRAC workflow, greatly simplifying the library preparation protocol from FFPE tissue samples.
Products
Kits
| Part Number | Product Name | Description |
| 520136 |
truXTRAC FFPE DNA microTUBE Kit -Column Purification (25) |
The kit contains reagents and consumables required for the high yield and high quality DNA extraction and purification from 25 FFPE tissue samples. Optimized to work with FFPE sections ≤ 25 μm containing ≤5 mg of tissue. |
Accessories
Centrifuge and Heat block microTUBE Adapters (quantity 10, PN 500406): Heat Block microTUBE Adapters are designed to fit a microTUBE into a heating block designed for 1.5 or 2 mL microcentrifuge tubes. It is required for Proteinase K incubation and to reverse formaldehyde crosslinks.
Holders and Racks
| REQUIRED PARTS | |||||||
| truXTRAC FFPE DNA microTUBE Kit – Column Purification (25) (PN 520136)
truXTRAC FFPE DNA microTUBE Kit – Bead Purification (24) (PN 520210) truXTRAC FFPE RNA microTUBE Kit – Column Purification (25) (PN 520161) truXTRAC FFPE total NA kit – column purification (25) (PN 520220) truXTRAC FFPE DNA microTUBE Kit for chemagen Technology (24) (PN 520176) |
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| Focused-ultrasonicator | LE220 | E220 & E210 | E220 evolution | S-Series | M220 | ME220 | |
| Rack/ Holder/ Insert | Rack-XT 24 Place microTUBE Screw-Cap(PN 500308) | Rack 24 Place microTUBE Screw-Cap (PN 500308) and Intensifier(PN500141) | Rack E220e 4 Place microTUBE Screw Cap(PN500432) and Intensifier(PN500141) | Holder microTUBE Screw-Cap(PN500339) | Holder XTUPN500414 & Insert XTUPN500489 or Holder-XT PN500358 * | ME220 Waveguide 4 Place PN500534
E220 Rack 4 place microTUBE screw-cap PN 500522 |
|
| Heat Block | Heat Block microTUBE Adapters (PN500406) | ||||||
| truXTRAC FFPE DNA 8 microTUBE Strip Kit for chemagen Technology (96) (PN 520147) | |||||||
| Focused-ultrasonicator | LE220 | E220 & E210 | E220 evolution | ||||
| Rack/ Holder/ Insert | Rack-XT 12 Place 8 microTUBE Strip V2(PN500485) | Rack 12 Place 8 microTUBE Strip V2 (PN500444) and Intensifier(PN500141) | Rack E220e 4 Place microTUBE Screw Cap(PN500432) and Intensifier (PN500141) | ||||
| Cap Press Tool | Cap Press Tool 8 microTUBE Strip V2 (PN500469) | ||||||
| Heat Block Rack | Heat Block Rack 12 Place 8 microTUBE Strip V2(PN500481) (x2) | Heat Block 8 microTUBE Strip V2 (PN500493) (x2) | |||||
* Compatible legacy part, now superseded by Holder XTU & Insert XTU microTUBE
Focused-ultrasonicators
Technical Literature
Protocols
- truXTRAC FFPE DNA microTUBE Kit – Column Purification (25) (pdf)
- truXTRAC FFPE DNA microTUBE Kit – Bead Purification (24) (pdf)
- truXTRAC FFPE DNA microTUBE Kit for chemagen Technology (24) (pdf)
- truXTRAC FFPE DNA 8 microTUBE Strip Kit for chemagen Technology (96) (pdf)
Application Notes
- Assessing FFPE DNA Quality: the “Illumina FFPE QC Kit” enables quantitation of improvements in FFPE DNA Extraction Technologies
- A comparison of active and passive FFPE DNA extraction methods and effects on downstream analysis metrics
- DNA Extraction from FFPE samples with Covaris Focused-ultrasonicators
FFPE Kit MSDS Information
Videos & Webinars
Webinar
NGS-quality RNA and DNA from FFPE tissues with a novel method of active paraffin removal
Movie
A short sequence showing paraffin emulsification from a 7 μm FFPE section
Frequently Asked Questions
Covaris provides tools and technologies to improve pre-analytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.
