The tissueTUBE, is a unique biomedical device constructed of strong, flexible inert plastic. When used in combination with a glass AFA tube, tissueTUBE devices facilitate the closed vessel cryofracture of a biological tissue sample in a very convenient, controlled, and easy to use manner. The tissueTUBE was designed for use with the CryoPrep™ tissueTUBE™ Impactor to ensure optimum performance with every sample. The film used in the tissueTUBE is made of Kapton, a “space-age” polymer originally developed to withstand the extreme temperature fluxes experienced in orbit. It is possible to snap-freeze a tissue sample in liquid nitrogen directly in the tissueTUBE, and because the film is so thin, the heat transfer from the sample is extremely fast, (e.g., 3 times faster than freezing in a cryo vial). Furthermore, the resilience allows for reliable cryofracture with the CryoPrep Systems, even at cryogenic temperatures. The tissueTUBE may be used for long term cryogenic storage by utilizing the tissueTUBE plugs.
Combining the tissueTUBE with a glass AFA Tube allows for the seamless integration of tissue cryofracture with a CryoPrep tissueTUBE Impactor and AFA facilitated chemical stabilization and homogenization.
Several sizes of tissueTUBE Devices are available to accommodate different tissue masses. For especially hard samples, extra thick (XT) Kapton film tissueTUBE Devices are available.
No sample-to-sample cross contamination
No cleanup required
No aerosol risk
The benefits of tissueTUBE Devices enhance the yield and fidelity of extraction of all chemicals, biomolecules, and metabolites from medium to large tissue samples. To process smaller tissue samples (t-PREP Biomarker Extraction System. Below are links to more information on the most common applications tissueTUBE Devices are used in.
If you are interested in applying the se products to advanced tissue processing applications not listed here please contact Covaris for assistance in developing your protocols.
The benefits of tissueTUBE Devices enhance the yield and fidelity of extraction of chemicals, biomolecules, and metabolites from medium to large tissue samples. To process smaller tissue samples (t-PREP Biomarker Extraction System.
If you are interested in applying Covaris technologies to your specific application please contact us.
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PMCID: PMC2782329 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782329/?tool=pubmed