The oneTUBE is made of a unique polymer for low impedance and better transmission of acoustic energy. The material focuses and efficiently couples acoustic energy and optimizes cavitation, resulting in the tight size distribution of DNA fragments required for NGS workflows. This unique configuration generates uniformly distributed cavitation facilitating an unprecedented standardized sample processing, which in turn increases the efficiency and reproducibility of nucleic acid shearing. The new plate format also allows for use as typical labware with compatibility with heat blocks, magnets and thermocyclers. It can easily be used on an automation platform in an NGS workflow improving turnaround time and throughput. With no transfer steps necessary, a complete library prep can be run in oneTUBE reducing cost of consumables and any sample loss.