Optimized Covaris truXTRAC® FFPE RNA Protocol for Isolating RNA from Laser-Capture Microdissected (LCM) FFPE Tissue for Next Generation Sequencing

SUMMARY
It is well characterized that RNA extraction from FFPE tissues can be challenging, often producing low yields and/or poor-quality RNA which can adversely affect success of downstream applications such as Next-Generation Sequencing (NGS). In this application note we describe an optimized workflow using Covaris truXTRAC FFPE RNA kits with deparaffinized, stained LCM-FFPE tissues of cancerassociated stroma (CAS) and matched normal stroma derived from canine mammary tumors. Using this adapted truXTRAC workflow, compared to the classical protease-based RNA isolation methods, total RNA yields were increased by 8- to 12-fold and RT-qPCR Cq values were 2.3-fold lower on average, signifying better performance. Finally, this improved quantity and quality of RNA performs favorably well in NGS workflows, overcoming the challenges of using low input RNA in RNA sequencing.

A high-efficiency cellular extraction system for biological proteomics

Journal of Proteome Research, 2015, 14 (8), pp 3403–3408

Dhabaria et. al.

The purpose of the study was to develop a general approach for native proteome extraction, the authors sought to identify cellular disruption methods that maximize the number and variety of extracted proteins while preserving their native functional activities.

Covaris, Inc. and OmniSeq, LLC Today Announced truXTRAC™ FFPE DNA Extraction and Purification Kit as the Method of Choice for Obtaining Clinical-grade DNA from FFPE Samples for Use with OmniSeq Target℠ Testing

Woburn, MA  – September 1st, 2015 – truXTRAC is a comprehensive novel solution for clinical-grade DNA extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) tissues utilizing Covaris Adaptive Focused Acoustics® (AFA™) technology. The unique focused, short-wavelength acoustic field generated by AFA enables rapid, active paraffin removal and rehydration of FFPE tissue samples in aqueous buffer, thus enabling efficient and rapid tissue digestion, crosslink reversal, and nucleic acid release.

Covaris, Inc. and OmniSeq, LLC Today Announced truXTRAC™ FFPE DNA Extraction and Purification Kit as the Method of Choice for Obtaining Clinical-grade DNA from FFPE Samples for Use with OmniSeq Target℠ Testing

Woburn, MA  – September 1st, 2015 – truXTRAC is a comprehensive novel solution for clinical-grade DNA extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) tissues utilizing Covaris Adaptive Focused Acoustics® (AFA™) technology. The unique focused, short-wavelength acoustic field generated by AFA enables rapid, active paraffin removal and rehydration of FFPE tissue samples in aqueous buffer, thus enabling efficient and rapid tissue digestion, crosslink reversal, and nucleic acid release.