Combining Covaris 96 oneTUBE-10 AFA Plate with Swift Biosciences Accel-NGS 2S Kit for an Optimized Single Tube Workflow for Robust, Low Input NGS Library Preparation

In this case study, DNA shearing in the oneTUBE was paired with Swift Biosciences™ Accel-NGS 2S® Kit for NGS library construction. The
2S kit preserves complexity from low input samples due to enhanced end repair chemistry for highly efficient adapter ligation in a single tube
workflow. This combination enables for the first time DNA shearing, end repair, and adapter ligation in the same vessel, thereby eliminating
cumbersome sample transfers. We present sequencing data from libraries constructed with this workflow and compare them to libraries
constructed according to standard “with transfer” protocol in microTUBE.

Optimized Covaris truXTRAC® FFPE RNA Protocol for Isolating RNA from Laser-Capture Microdissected (LCM) FFPE Tissue for Next Generation Sequencing

SUMMARY
It is well characterized that RNA extraction from FFPE tissues can be challenging, often producing low yields and/or poor-quality RNA which can adversely affect success of downstream applications such as Next-Generation Sequencing (NGS). In this application note we describe an optimized workflow using Covaris truXTRAC FFPE RNA kits with deparaffinized, stained LCM-FFPE tissues of cancerassociated stroma (CAS) and matched normal stroma derived from canine mammary tumors. Using this adapted truXTRAC workflow, compared to the classical protease-based RNA isolation methods, total RNA yields were increased by 8- to 12-fold and RT-qPCR Cq values were 2.3-fold lower on average, signifying better performance. Finally, this improved quantity and quality of RNA performs favorably well in NGS workflows, overcoming the challenges of using low input RNA in RNA sequencing.

Evaluation of Next Generation Library Preparation using DNA Extracted from Dried Blood Spots using truXTRAC® DBS DNA kit

Dried Blood Spots (DBS) provide an easy and inexpensive way to collect and store peripheral blood specimens from infants, children nd adults.[1] The use of DBS allows for less invasive procedures for patients and easier shipment while still providing the ability to run molecular or clinical biochemical assays. This convenient method for the long term room-temperature storage of materials also minimizes storage and archival space. The truXTRAC DBS DNA kit is designed for controlled and efficient extraction of next-generation sequencing (NGS)-grade DNA from DBS samples using Adaptive Focused Acoustics® (AFA®). Covaris AFA enables sample rehydration while providing simultaneous cell lysis and controlled mechanical DNA shearing, resulting in high-yield, high-quality and NGS library preparation ready DNA.

A high-efficiency cellular extraction system for biological proteomics

Journal of Proteome Research, 2015, 14 (8), pp 3403–3408

Dhabaria et. al.

The purpose of the study was to develop a general approach for native proteome extraction, the authors sought to identify cellular disruption methods that maximize the number and variety of extracted proteins while preserving their native functional activities.

Covaris, Inc. and OmniSeq, LLC Today Announced truXTRAC™ FFPE DNA Extraction and Purification Kit as the Method of Choice for Obtaining Clinical-grade DNA from FFPE Samples for Use with OmniSeq Target℠ Testing

Woburn, MA  – September 1st, 2015 – truXTRAC is a comprehensive novel solution for clinical-grade DNA extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) tissues utilizing Covaris Adaptive Focused Acoustics® (AFA™) technology. The unique focused, short-wavelength acoustic field generated by AFA enables rapid, active paraffin removal and rehydration of FFPE tissue samples in aqueous buffer, thus enabling efficient and rapid tissue digestion, crosslink reversal, and nucleic acid release.

Covaris, Inc. and OmniSeq, LLC Today Announced truXTRAC™ FFPE DNA Extraction and Purification Kit as the Method of Choice for Obtaining Clinical-grade DNA from FFPE Samples for Use with OmniSeq Target℠ Testing

Woburn, MA  – September 1st, 2015 – truXTRAC is a comprehensive novel solution for clinical-grade DNA extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) tissues utilizing Covaris Adaptive Focused Acoustics® (AFA™) technology. The unique focused, short-wavelength acoustic field generated by AFA enables rapid, active paraffin removal and rehydration of FFPE tissue samples in aqueous buffer, thus enabling efficient and rapid tissue digestion, crosslink reversal, and nucleic acid release.

PerkinElmer and Covaris Offer Next Gen Sequencing Grade Nucleic Acid Purification and Automated Sample Prep from FFPE Tissue Samples

PerkinElmer’s chemagicTM research instrumentation and kits are now available with the Covaris “truXTRAC FFPE DNA microTUBE Kit for chemagen Technology” for DNA/RNA purification powered by Adaptive Focused Acoustics® (AFA™) technology.