Covaris Introduces the 96 oneTUBE-10 AFA Plate for Improved NGS Workflows

Woburn, MA – November 14, 2017 – Today, Covaris Inc. announced the release of the 96 oneTUBE-10 AFA Plate, which enables scalable and simplified workflows by leveraging Covaris’ gold standard focused-acoustic technology to meet rapidly increasing Next-Generation Sequencing (NGS) demands for high-throughput and low-input library preparation.  By using the oneTUBE-10 offering, transfer steps within the NGS library prep workflow have been eliminated from mechanical DNA shearing (using Covaris’ Adaptive Focused Acoustics® (AFA®) technology) through end repair, A-tailing, ligation, and SPRI clean-up.  The oneTUBE-10 product is specifically designed for use with Covaris’ AFA technology for DNA shearing at volumes ranging from 10 to 50 µL, achieving highly accurate and reproducible results.

This new AFA vessel, is made of an engineered polymer for low impedance and efficient transmission of acoustic energy.  oneTUBE-10 effectively controls acoustic cavitation to enable reproducible and precisely-tuned hydrodynamic shear forces.   Designed to SLAS standards, oneTUBE-10 is compatible with standard heat blocks, magnets, and thermocyclers.  oneTUBE-10 can be used on any liquid handling platform, providing a universal solution for streamlining NGS workflows.  Paired with the LE220-plus (Covaris’ newest Focused-ultrasonicator), NGS workflows are easily standardized by integration with high-throughput laboratory automation.

Covaris Founder and CEO, Jim Laugharn, said, “oneTUBE-10 is a game-changer for NGS workflows.  The combination of the Covaris AFA-energetics, the process behind the industry gold standard for robust, random, and unbiased DNA shearing, with a uniquely engineered polymer consumable enables highly automated library preparation, nucleic acid extraction, and additional applications in a single tube.  The improved workflow possibilities are unlimited!”

Future applications of oneTUBE-10 include FFPE extraction, enhanced ligation enzymatic reactions, and other biomolecular processes utilizing the Covaris technology.  AFA can now be applied at various steps including bead binding, mixing, and elution, while scaling down to low volumes.  Additionally, oneTUBE-10 product iterations will include an 8-strip format and will scale up to 384 well formats and beyond.

 

About Covaris, Inc.

Covaris is a recognized industry innovator in preanalytical sample preparation workflows involving sample stabilization, extraction, and processing.  Leveraging its patented Adaptive Focused Acoustics (AFA) technology and novel engineering solutions, Covaris has developed comprehensive packages of instrumentation, applications, reagents, and consumables to address the increasing sensitivity requirements of analytical technologies such as NGS and mass spectrometry.  Covaris Focused-ultrasonicators are recommended by all major NGS platform providers, and are used by leading Genome Centers worldwide.  The Covaris AFA process is extensively cited in peer-reviewed research articles on DNA fragmentation, ChIP, FFPE nucleic acid extraction, metabolomics, small molecule extraction, proteomics, and nanoemulsion formulation.  For more information, please visit: www.covaris.com.

For additional information, please contact: Paulina Kocjan, pkocjan@covaris.com

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.

Carl Beckett VP of Business Development will be discussing how AFA can control nucleation and crystal distribution on June 21 at the Cambridge R&D Process Development Seminar.

R&D Process Development Seminar
Symposium focusing on R&D Process Development

June 21 @ 8:00 am5:00 pm in Cambridge, MA

Adaptive Focused Acoustics Processing to Aid Recovery of Fungi from CF Patient Sputum Samples

Background: Cystic fibrosis (CF) is a genetic disorder that afflicts over 70,000 people worldwide. It is characterized by viscous mucus that accumulates in the lungs creating challenges for microbiology labs processing sputa toisolates lower growing fungal species. Our laboratory attempted to liquefy and homogenize mucoid sputum samples by exposure to Adaptive Focused Acoustics™ (AFA) in order to increase recovery of fungal pathogens.

H.B. Miller, et al

Covaris, Inc. and OmniSeq, LLC Today Announced truXTRAC™ FFPE DNA Extraction and Purification Kit as the Method of Choice for Obtaining Clinical-grade DNA from FFPE Samples for Use with OmniSeq Target℠ Testing

Woburn, MA  – September 1st, 2015 – truXTRAC is a comprehensive novel solution for clinical-grade DNA extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) tissues utilizing Covaris Adaptive Focused Acoustics® (AFA™) technology. The unique focused, short-wavelength acoustic field generated by AFA enables rapid, active paraffin removal and rehydration of FFPE tissue samples in aqueous buffer, thus enabling efficient and rapid tissue digestion, crosslink reversal, and nucleic acid release.

Covaris, Inc. Opens New Office in Europe

Covaris, Inc., an innovator in the life science tools and the clinical diagnostic market spaces, announced today that its European subsidiary, Covaris Ltd, which is located in the United Kingdom, will commence
operations on May 1, 2014.

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